Fig. 4. Immunofluorescence confocal micrographs showing subcellular dUNC-45 localization in the body-wall muscle of third instar wild-type larvae. (A) α-Actinin staining highlights the location of the Z-discs. (B) dUNC-45 is discretely localized in the sarcomere. (C) Actin staining with phalloidin shows the location of I bands in the sarcomeres. (D) dUNC-45 colocalizes with α-actinin (bright pink in the overlay panel) and bisects the actin-containing I bands, which supports dUNC-45 location in the Z-discs of the sarcomeres.
Drosophila UNC-45 accumulates in embryonic blastoderm and in muscles, and is essential for muscle myosin stability (2011) Journal of Cell Science (124):5, 699-705